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The JGI’s DNA Synthesis Program supports a wide range of projects, … to drive innovations that align with DOE's missions. … The DNA Synthesis Program plays a central role in advancing … of life, in a uniquely integrated context.  … The MycoCosm web portal provides data access, visualization, and analysis …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … … are several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… and other omics technologies.  The exponential growth of DNA sequencing data, coupled with recent computational … Neri U. et al. (2022) Expansion of the global RNA virome reveals diverse clades of bacteriophages. Cell . … Commun. 7:10476 Varghese NJ,  et al. (2015) Microbial species delineation using whole genome sequences. Nucleic …

The DNA Synthesis Science User Advisory Committee is chartered …

…  The recent updates were approved by the DOE to address the DNA Synthesis program. When fully executed, the new User … User Agreement , JGI User Agreement for Federal Agencies , DNA Synthesis Appendix  and DNA Sequencing Appendix are available for download.)  After …

… elements. Beyond exploring and characterizing microbial diversity, work in the Metagenome Program uses several … functions that shape ecosystems. By decoding the microbial DNA in natural environments, the Program provides a snapshot … We develop computational tools for analyzing viral fragments via IMG/VR and methods to capture  environmental …

The JGI has diverse capabilities in: DNA and RNA sequencing, Sample and library preparation, DNA … annotation (single organism). Note: smRNA sequencing is no longer supported. For annotation: de novo assembly. For … profiling (single organism). Note: smRNA sequencing is no longer supported. For counting: text file of gene counts …

… provided by JGI and the JGI user community below for DNA preparation tools, high molecular-weight DNA extraction and RNA preparation.  JGI sample QC protocols can be found here . DNA Extraction Protocols: … Tips for High Molecular Weight …

… facing humanity. A major focus are biofuel feedstock species and plant-microbe interactions, advancing efforts to … the globe to identify genes to improve our domesticated species.  The program supports the development of … for key plant species, enhancing our understanding of plant diversity and integrating various data types to explore the …

I talked about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, … of those 150 plus people. I have to admit, as a PI, I’m no longer impressed by that number. But as a postdoc, I … family would be a set of gene clusters across different species, which all encode the production of either the same …

Review JGI sample QC requirements and protocols Obtaining DNA and RNA of suitable quantity and quality has been the … project. It is imperative that users properly assess DNA or RNA mass prior to shipment to the  JGI. The documents … metadata is collected via spreadsheet or directly via a web interface prior to shipping samples to the JGI. PI sends …

… and computational exploration of uncultivated viral diversity, characterization of molecular mechanisms involved …   19 , 407 Berg, M. et al. (2021) Host population diversity as a driver of viral infection cycle in wild …   15 , 1569–1584 Roux, S. et al. (2019) Cryptic inoviruses revealed as pervasive in bacteria and archaea across Earth’s …