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… Fine-tuning metabolic gene expression helps identify variants in yeast genes. The … area in the DNA, to determine which targets might benefit from an intermediate level of expression. Similar to the … under FP00005685. In addition, M.D. acknowledges funding from a National Defense Science and Engineering Graduate …

… our interview this week is with Professor Brian Bachmann from Vanderbilt University’s Chemistry Department, where he … It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … if you want– if you really want to, you can do heterologous expression. You can do homologous expression techniques and …

… The JGI’s Metabolomics Program works to uncover the roles of small molecules in biological … metabolomics fuels the discovery  of novel metabolites, genes, and proteins. It also enables exploration of energy and nutrient exchange, as well as microbial and … JGI scientists are able to link metabolic activity with gene expression, providing deeper insights into functional …

… we get to present our conversation with Marnix Medema , from Wageningen University in the Netherlands. Marnix is … and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene cluster families. And he tells us about forthcoming … was a proposal on synthetic biology of engineering gene expression in bacteria – however, the professor was actually …

… you’ll be hearing about microbiology and natural products from the Great Salt Lake, and you’re going to learn, like … bit more into fungi and learning the differences of all the genetic differences and then expanded on on that knowledge … it was amazing. And now you have– just to the exponential expression of all these genomes and what can happen with all …

… lot going on, so I took a little break, but I’m hoping that from here I can get back to a regular schedule. I’m going to … my undergraduate in microbiology and then PhD in microbial genetics in the UK. And came across to the US for a couple … explore the regulation of biosynthetic gene cluster expression in organisms. And that’s led by Hiroshi Otani …

… Our research is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and … data. We use multi-omics (metagenomics, metatranscriptomics, single cell genomics and phylogenomics) and machine … complexity, and encode for a treasure trove full of novel genes ready to be tapped for downstream applications. …

… over our interview with Dr Elizabeth (or Betsy) Parkinson from Purdue University. She’s in the Department of Chemistry … oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … that you can turn on the natural product, say clusters or expression systems so that-- BETSY PARKINSON: Exactly. DAN: …

…   The JGI’s Secondary Metabolites Program focuses on the exploration of secondary metabolites across bacteria, … for fundamental growth, provide critical advantages to the organisms producing them, and are often involved in … conditions, with only a small portion of the biosynthetic gene clusters (BGCs) being expressed. The program works to …

… conversation with Aaron Puri. He is an Assistant Professor from the Chemistry department at the University of Utah, so … and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … of these or any organisms. So developing good heterologous expression hosts like maybe in many fields now like in …

… about natural products and the science and scientists of secondary metabolism. Welcome back for our second season of … genome mining on more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find … ALISON TAKEMURA: I know that it’s looking in the genome for genetic elements of interest, you know, something like …

… about natural products and the science and scientists of secondary metabolism. Welcome back. This will be episode 12 of … Prodcast. And it’s our conversation with Nadine Ziemert from the University of Tübingen in Germany. Like me, she’s a … of it. So it wasn’t only chemistry or only biochemistry or genetics. It was also ecology. It was also evolution. It was …