Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA
As the focus for CRISPR-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one gRNA per target; however, this complicates construct assembly and increases the possibility of off-target mutations. In this study, we… [Read More]