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… usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … to take a little bit of time. So we are starting with doing DAP-seq and we're hoping I think to get the DAP-seq done in … to Medicines outreach program JGI Release on DAP-Seq: Enlarging Windows into Understanding Gene Functions Purdue's …

… always wanted to write a book but it’s really hard to break into that area. I mean you have to secure an agent and … things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … educational initiatives and work with school children and understanding the plants and their environment. We also were …

… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … Secondary Metabolites Science Program, which I was hired into, and my job is to do cool research, and also to talk to … in a cell or a living creature that make it specialized or adapted to its environment. Chemical- chemistry-wise. So it’s …

… and you can still make more of it, then you start going into human trials or animal trials first, and going through … and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … in terms of – well it’s really simple in terms of understanding it because it’s an assembly line. You just go …

… thought I’d take an academic job then, but I ended up going into industry. Saw a job at Roche, and that was to work with … MOUNCEY: Taking a bit of risk and then actually really adapting from there to dial in conditions and things. I think … NIGEL MOUNCEY: Oh, sure. I think our legacy obviously is in sequencing.  DAN: Of course.  NIGEL MOUNCEY: Sequencing the …

… basic ideas of the science of natural products, and we dive into the very early history of the field. If you continue … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have … changes that nature has made. So secondary metabolism is adaptation of primary metabolism. They’re duplications of the …

I wanted to start, maybe just by asking you how did you get into the field of natural products?   NANCY: Yeah, that’s a … After I got my undergraduate degree, which was in general biology, I joined the Peace Corps. And when I was … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   …

… have to see if I’m going to slip a day on releasing it into the feeds, but I’m trying, you guys. Schedules are … of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … for the project. DAN: Do you see broader applications for understanding these systems? AARON PURI: Yeah. Are you …

… work we’ve determined that the ANMG deposits those bacteria into the jelly coat of the eggs. And so these eggs are laid … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … Have been in the last several years figuring out how to adapt what we want to get out of the mass spectrometer to …

… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … episode with her in the near future where we can dive into that. And I’m also dying to talk in more detail about … in everninomicin and analogs of that, you’re really understanding one specific biosynthetic pathway. I guess how …

So maybe we could start with that background – what got you into secondary metabolism, besides being forced to work with … at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … for many millions, sometimes billions, of years. And understanding how those changes that evolution brokers are …

… started collaborating with Ben and his group to work on sequencing, synthesis and genome mining technology to help … he has an accent, and, so, I don’t really have any trouble understanding him, but I know from the download stats that … for every episode we put out. I actually put a lot of time into preparing those transcripts, so I hope somebody’s going …