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… metabolism. Hey everybody, this is Dan with episode 13 of National Prodcast. This continues our little section … At the end of the day, all the content produced by microbes the result of millions of years of evolution. And … the active site of an enzyme. So there is no promiscuity, generally speaking, in primary metabolism. Nevertheless, …

… about microbiology and natural products from the Great Salt Lake, and you’re going to learn, like me, what a “haboob” … bit more into fungi and learning the differences of all the genetic differences and then expanded on on that knowledge … there a way to do that? DAN UDWARY: Well, so there’s good news and bad news on that. So one of the things that we’re …

… of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use … complexity, and encode for a treasure trove full of novel genes ready to be tapped for downstream applications. … Understanding the complex relationships between microbes and eukaryotes and their impact on ecosystem …

… and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … put into antiSMASH after downloading it was isolated from Lake Washington. So it’s really interesting that it was … ecology community to be able to better predict how their microbes are interacting with each other from sequence …

… my undergraduate in microbiology and then PhD in microbial genetics in the UK. And came across to the US for a couple … firstly set up a baseline process. And then really dig deeper into both aspects of the strain, as well as the … But they really span our core science programs which are microbes, and fungi, and algae plants, synthetic biology. …

… inside them. And this was, I think it must’ve been about 2001, maybe, and so it was before smartphones and before … I was imagining some massive contraption for amplifying genes. I wasn’t really sure what actinobacteria were. Dan … what’s important in terms of the chemistry produced by microbes. And by what’s important, I mean, like, where …

… metabolomics fuels the discovery  of novel metabolites, genes, and proteins. It also enables exploration of energy … processes, development of high performance plants and microbes, and understanding microbiome interactions. The  … JGI scientists are able to link metabolic activity with gene expression, providing deeper insights into functional …

… technology to help access complicated natural product gene clusters, and we’re all really excited about that, and … called Mylotag. Wyeth was later acquired by Pfizer in 2009. So, a simple mistake, but I’m happy to be able to … So I wanted to ask you also about – because you were in the news recently – TSRI has acquired the Pfizer strain …

… especially for these primer episodes, if you want to dive deeper on all this at http://naturalprodcast.com . And then … ALISON TAKEMURA: I know that it’s looking in the genome for genetic elements of interest, you know, something like … we’re going to add another 87,000. So that’s, what, a 20%, 30% increase?  Dan insert: It’s a 21.75% increase. ALISON …

… in the feed, the secondary metabolism field has been dominated for … well, as long as I can remember, by … After I got my undergraduate degree, which was in general biology, I joined the Peace Corps. And when I was … use natural products necessarily. Fungi are large. They’re microbes, but they’re large microbes. And they have all …

… oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … about 140 unique cyclic peptides. And from that, about 130 of them were not previously known. DAN: Very cool. … fun that we've started that is a different subject is from microbes to medicine labs. So this is-- we've been working …

… It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … have to do a 10,000-fold dilution to plate that out. The deeper you go in the cave, the less dilution. You can be … them on a regular basis. So they are full of terrestrial microbes. So they’re open ecosystems. They’re not sealed off …