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    Data yielded from RIViT-seq increased the number of sigma factor-gene pairs confirmed in Streptomyces coelicolor from 209 to 399. Here, grey arrows denote previously known regulation and red arrows are regulation identified by RIViT-seq; orange nodes mark sigma factors while gray nodes mark other genes. (Otani, H., Mouncey, N.J. Nat Commun 13, 3502 (2022). https://doi.org/10.1038/s41467-022-31191-w)
    Streamlining Regulon Identification in Bacteria
    Regulons are a group of genes that can be turned on or off by the same regulatory protein. RIViT-seq technology could speed up associating transcription factors with their target genes.

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    (PXFuel)
    Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
    In a special issue of the journal Synthetic Biology, JGI scientific users share how they’ve worked with the JGI DNA Synthesis Science Program and what they’ve discovered through their collaborations.

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    A genetic element that generates targeted mutations, called diversity-generating retroelements (DGRs), are found in viruses, as well as bacteria and archaea. Most DGRs found in viruses appear to be in their tail fibers. These tail fibers – signified in the cartoon by the blue virus’ downward pointing ‘arms’— allow the virus to attach to one cell type (red), but not the other (purple). DGRs mutate these ‘arms,’ giving the virus opportunities to switch to different prey, like the purple cell. (Courtesy of Blair Paul)
    A Natural Mechanism Can Turbocharge Viral Evolution
    A team has discovered that diversity generating retroelements (DGRs) are not only widespread, but also surprisingly active. In viruses, DGRs appear to generate diversity quickly, allowing these viruses to target new microbial prey.

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    Photograph of a stream of diatoms beneath Arctic sea ice.
    Polar Phytoplankton Need Zinc to Cope with the Cold
    As part of a long-term collaboration with the JGI Algal Program, researchers studying function and activity of phytoplankton genes in polar waters have found that these algae rely on dissolved zinc to photosynthesize.

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    This data image shows the monthly average sea surface temperature for May 2015. Between 2013 and 2016, a large mass of unusually warm ocean water--nicknamed the blob--dominated the North Pacific, indicated here by red, pink, and yellow colors signifying temperatures as much as three degrees Celsius (five degrees Fahrenheit) higher than average. Data are from the NASA Multi-scale Ultra-high Resolution Sea Surface Temperature (MUR SST) Analysis product. (Courtesy NASA Physical Oceanography Distributed Active Archive Center)
    When “The Blob” Made It Hotter Under the Water
    Researchers tracked the impact of a large-scale heatwave event in the ocean known as “The Blob” as part of an approved proposal through the Community Science Program.

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    A plantation of poplar trees. (David Gilbert)
    Genome Insider podcast: THE Bioenergy Tree
    The US Department of Energy’s favorite tree is poplar. In this episode, hear from ORNL scientists who have uncovered remarkable genetic secrets that bring us closer to making poplar an economical and sustainable source of energy and materials.

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    HPCwire Editor's Choice Award (logo crop) for Best Use of HPC in the Life Sciences
    JGI Part of Berkeley Lab Team Awarded Best Use of HPC in Life Sciences
    The HPCwire Editors Choice Award for Best Use of HPC in Life Sciences went to the Berkeley Lab team comprised of JGI and ExaBiome Project team, supported by the DOE Exascale Computing Project for MetaHipMer, an end-to-end genome assembler that supports “an unprecedented assembly of environmental microbiomes.”

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    With a common set of "baseline metadata," JGI users can more easily access public data sets. (Steve Wilson)
    A User-Centered Approach to Accessing JGI Data
    Reflecting a structural shift in data access, the JGI Data Portal offers a way for users to more easily access public data sets through a common set of metadata.

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    Phytozome portal collage
    A More Intuitive Phytozome Interface
    Phytozome v13 now hosts upwards of 250 plant genomes and provides users with the genome browsers, gene pages, search, BLAST and BioMart data warehouse interfaces they have come to rely on, with a more intuitive interface.

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    screencap from Amundson and Wilkins subsurface microbiome video
    Digging into Microbial Ecosystems Deep Underground
    JGI users and microbiome researchers at Colorado State University have many questions about the microbial communities deep underground, including the role viral infection may play in other natural ecosystems.

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    Yeast strains engineered for the biochemical conversion of glucose to value-added products are limited in chemical output due to growth and viability constraints. Cell extracts provide an alternative format for chemical synthesis in the absence of cell growth by isolating the soluble components of lysed cells. By separating the production of enzymes (during growth) and the biochemical production process (in cell-free reactions), this framework enables biosynthesis of diverse chemical products at volumetric productivities greater than the source strains. (Blake Rasor)
    Boosting Small Molecule Production in Super “Soup”
    Researchers supported through the Emerging Technologies Opportunity Program describe a two-pronged approach that starts with engineered yeast cells but then moves out of the cell structure into a cell-free system.

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    These bright green spots are fluorescently labelled bacteria from soil collected from the surface of plant roots. For reference, the scale bar at bottom right is 10 micrometers long. (Rhona Stuart)
    A Powerful Technique to Study Microbes, Now Easier
    In JGI's Genome Insider podcast: LLNL biologist Jennifer Pett-Ridge collaborated with JGI scientists through the Emerging Technologies Opportunity Program to semi-automate experiments that measure microbial activity in soil.

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    A view of the mangroves from which the giant bacteria were sampled in Guadeloupe. (Hugo Bret)
    Giant Bacteria Found in Guadeloupe Mangroves Challenge Traditional Concepts
    Harnessing JGI and Berkeley Lab resources, researchers characterized a giant - 5,000 times bigger than most bacteria - filamentous bacterium discovered in the Caribbean mangroves.

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    In their approved proposal, Frederick Colwell of Oregon State University and colleagues are interested in the microbial communities that live on Alaska’s glacially dominated Copper River Delta. They’re looking at how the microbes in these high latitude wetlands, such as the Copper River Delta wetland pond shown here, cycle carbon. (Courtesy of Rick Colwell)
    Monitoring Inter-Organism Interactions Within Ecosystems
    Many of the proposals approved through JGI's annual Community Science Program call focus on harnessing genomics to developing sustainable resources for biofuels and bioproducts.

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    Coloring the water, the algae Phaeocystis blooms off the side of the sampling vessel, Polarstern, in the temperate region of the North Atlantic. (Katrin Schmidt)
    Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
    As warm-adapted microbes edge polewards, they’d oust resident tiny algae. It's a trend that threatens to destabilize the delicate marine food web and change the oceans as we know them.

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News & Publications
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July 22, 2013

Leaf-cutter ants project in Milwaukee Journal Sentinel

“These bacteria and these fungi have evolved for millions of years to deconstruct plant biomass,” said Frank Aylward, a graduate student at the University of Wisconsin-Madison who studies leaf-cutter ants and their fungus gardens. “We should try to learn from them and find out how it occurs in nature.” Read more at JSOnline.com about this… [Read More]

July 15, 2013

Microbial dark matter study in The Scientist

“Its scale and impact are exactly what Nature is there for,” [said Argonne National Laboratory’s Jack Gilbert.] “The data allow us to confirm some known relationships, but also uncovered some awesome new ones.” Read more in The Scientist [Read More]

July 15, 2013

Microbial dark matter study in Nature

“This is an astounding paper,” says Norman Pace, a microbiologist at the University of Colorado–Boulder. “The achievement of hundreds of genome sequences from single cells at a shot is an entirely new level of microbiology.” Read more in Nature [Read More]

July 15, 2013

Microbial dark matter study in BBC News

“For almost 20 years now we have been astonished by how little there is known about massive regions of the tree of life. This project is the first systematic effort to address this enormous knowledge gap.” – Phil Hugenholtz, director of the Australian Centre for Ecogenomics at the University of Queensland, in Australia Read more in… [Read More]

July 15, 2013

Microbial dark matter study in The Globe and Mail

“Sakinaw Lake’s hidden depths have become part of a massive effort to explore some of the least understood branches of the tree of life. “It’s a really interesting place to look for exotic microorganisms,” said Steven Hallam, an environment genomicist at the University of British Columbia.” Read more in The Globe and Mail [Read More]

July 15, 2013

Microbial dark matter project in Science

“Three years ago, Tanja Woyke, a microbiologist at the Joint Genome Institute in Walnut Creek, California, and colleagues decided to head into this uncharted territory by applying a newly developed sequencing approach to bacteria and archaea. Until recently, determining a genome’s makeup required many copies of the DNA, and thus only microbes grown in the… [Read More]

June 18, 2013

Ehux pangenome project in San Diego Union-Tribune

The algae are the third most abundant phytoplankton, and are a key component of the ocean food chain, nourishing animals including crustaceans, shellfish and other filter feeders.They’re characterized by their intricate shells, composed of interwoven lattices of calcium carbonate.“We kind of think of them as flowers of the ocean,” Read said. Read the full story… [Read More]

June 14, 2013

Ehux pangenome project in redOrbit

 “The Ehux genome is incredibly variable,” said study co-author Dr. Uwe John and biologist with the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research (AWI). “For example, if the genetic information of two humans is compared, an agreement of about 99 per cent is found. However, if, for example, we take two Ehux strains from… [Read More]

May 20, 2013

DOE Early Career Awardee’s work to involve DOE JGI collaboration

O’Malley’s research, which she recently presented at the spring meeting of the American Chemical Society, involves the use of anaerobic gut fungi from horses, sheep, and other large herbivores to convert the cellulose in plants into sugars. Nature has evolved these fungi to break through lignin, a tough biopolymer that surrounds cellulose, and convert that… [Read More]

March 25, 2013

Peach genome project in Biofuels Digest

In California, the US Department of Energy Joint Genome Institute (DOE JGI) is working to isolate the “evergreen” locus in peaches, which extends the growing season of the plant. Said Daniel Rokhsar, head of the DOE JGI Eukaryotic Program, “In theory, it could be manipulated in poplar to increase the accumulation of biomass.” Peach genes… [Read More]
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