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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… facing humanity. A major focus are biofuel feedstock species and plant-microbe interactions, advancing efforts to … the globe to identify genes to improve our domesticated species.  The program supports the development of … manipulation in the laboratory. Both germplasm (sequenced mutants and natural accessions) and protocols …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … 3 million molecules to be discovered, I don’t think – BEN: Three million for the bacteria alone! Another 30 million for …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … Yes. Yeah. I mean, I am– yeah. I just got the position like three years, four years– four years ago I think. Yeah. Yeah, … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … quorum sensing allows you to do — generally there’s just three components in these acyl homoserine lactone based …

… can get a good feeling for what this podcast is. The first three episodes are what we’re calling a Primer. We wanted to … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … at MIT, and I studied microbial ecology. I studied Vibrio species in the ocean, so heterotrophic bacteria. Now, we …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … could be copper, but unless you don’t mess about with the three pairs of electrons that you need to do these … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … nutrient cycling and bioproduct applications.  The team has three subgroups: Synthetic Biology Lab : a user science … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … synthesis where our gold standard was, if you’re doing three reactions a day, like that’s a good level of …

… to help advance their science by generating data in three overarching categories: Sequencing, Synthesis and … through end-to-end automation, and further advancing sequence applications, single cell genomics, DNA synthesis, …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …