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… of Oceanography in San Diego. Bill is a pioneer of marine natural products, getting into the ocean and looking … learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … quality. Yeah, for sure. NADINE ZIEMERT: Yeah, Dan… it’s a marine actinomycete bacterium. So they look kind of like … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … then got funded to work for two years at the Max Planck for marine microbiology in Bremen, Germany. And I wanted to work …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … to user project design, assembly, and quality control Strain Engineering Group : a research & development group … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… product dioxin ubiquinone, which actually comes from a marine bacterium. And so, I was working on chemically … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… technology to help access complicated natural product gene clusters, and we’re all really excited about that, and I … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… wouldn’t it be cool to look at these– it was actually marine invertebrates there– but look at these things in the … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … in cell biology. 25 (6), 339-346 Schulz et al. (2015) Marine amoebae with cytoplasmic and perinuclear symbionts …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … just not enough time and money. I mean, we have a huge marine fungal project in collaboration with Bill Fenical at … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… expression and functional exploration.  The developed strains represent an additional phase of user-accessible … into a taxonomically diverse suite of microbial chassis strains for elevated opportunity for expression. Research …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … has to have a story. And it can be looking for drugs from marine organisms, in which case you get to go to exotic …