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… expression and functional exploration.  The developed strains represent an additional phase of user-accessible … into a taxonomically diverse suite of microbial chassis strains for elevated opportunity for expression. Research …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … U.S. Culture Collection Network (USCCN) to nominate their strains for sequencing, as long as they have a scientific … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … And I’m using gallons of tetrahydrofuran and methylene chloride to make milligrams, in the end, of compounds. And …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … major discovery from the genome sequencing of this Actino strains, instead of knowing some of the organism can produce …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … molecule of water. DAN UDWARY: Mhm. So oxygenases, they’re oxidizing other molecules. ALISON NARAYAN: Correct. Correct. …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … they’re obligate marine. There’s so far no species or no strains known that really grow in freshwater. But they’re … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

I hesitate to make a guess but maybe 50 to 100 different strains and they are in individual tubules.  And so these … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …