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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… most definitely does not have an ocean. Brad: I – that’s a desert.  Dan: Yeah. How does one do marine natural products … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … in natural products were there.  I’ve always wondered if deserts or maybe glaciers are places where there are less … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … processes at that point. Those are very– those are deserts. They’re very, very sparse areas. And we have …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … Program leads to discuss available options. Note: In 2021, we conducted an extensive internal product audit to … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

Weight DNA extraction and quality control (from PacBio) 2) Review guidelines detailing quantity and quality … must also include a completed Export Certification Form . 2) Obtain Approval for Shipping Before approval to ship is … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … at that time just think that the ocean was kind of like a desert? Or that certainly there was marine life, there were … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

Berkeley National Laboratory. The JGI consists of around 280 researchers and support staff in the Integrative …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …