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Sequencing Submission Requirements for DNA Synthesis
When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …
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Natural Prodcast Episode 15: Katherine Duncan
I want to talk about ActinoBase, we want to talk about Antarctica, and we want to talk about molecular networking, … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …
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Sequencing Technologies
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Natural Prodcast Episode 19: Bill Fenical
… of Oceanography in San Diego. Bill is a pioneer of marine natural products, getting into the ocean and looking … learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …
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Natural Prodcast Episode 12: Nadine Ziemert
… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … quality. Yeah, for sure. NADINE ZIEMERT: Yeah, Dan… it’s a marine actinomycete bacterium. So they look kind of like … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …
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Mission
Laura.Selbmann©PNRA. All the sampling activities in Antarctica have been performed in the frame of italian … in doing so enabled the JGI to be the first to publish the sequence analysis of the target chromosomes 5, 16, and 19, … of organics will not be considered. Projects targeting marine systems must clearly demonstrate relevance and …
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Natural Prodcast Episode 8: Eric Schmidt
… grade list of goals in life: on that list are being a marine biologist and being a chemist, and it’s actually … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …
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Products
… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …
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Natural Prodcast Episode 16: Marcy Balunas
… have done some work at the polls or is it the Arctic or Antarctic but you’ve gone to retrieve some bacteria that … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …
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How to Submit Sequencing Samples
… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …
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Who We Are
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Cultivating Symbiotic Antarctic Microbes
… organisms as had been originally thought. The Impact The Antarctic lakes are a “treasure trove” of unknown microbes … in understanding the genomic basis and ecological consequences of microbe-microbe interactions. Using metagenomes … native microbes. Metagenomes from enrichment cultures were sequenced and assembled by the JGI. From these assemblies, …
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