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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
A group led by JGI scientists have made strides in improving and applying a method that allows researchers … ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the genome. “CRAGE is central to our ability to offer host-engineering for our users,” says JGI Director …
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Year in Review: JGI’s 2018 Progress Report Available Now
The latest edition of the JGI Progress Report highlights notable research and scientific collaborations in 2018. The cover is an image of Mono Lake, a saline soda lake east of California’s …
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Understanding Wildfire Recovery, Starting in Soil
… a wildfire. The Science After a wildfire burns, recovery begins at the forest floor. Within the soil, a microbiome of bacteria, viruses and fungi process carbon and nitrogen, … the microbes within the soil. To get a clearer picture of how burned ecosystems return to health, researchers have …
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Ray Turner Completes Last Tour of Lab Duty at JGI
… last 15 years, Ray Turner has been one constant for the Joint Genome Institute. On July 1, 2020, that all changed. … as a typical monthly JGI All-Hands Meeting, after a couple of perfunctory items, the agenda went off the rails – according to plan but unbeknownst to Ray and …
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Toward More Reproducible, Reusable Stable Isotope Probing Experiments
… communities than ever before — especially with the prospect of meta-analyses boosted by AI and Machine Learning — JGI researchers and collaborators work to … communities. By pairing RNA or DNA sequencing with tracings of stable, heavy isotopes, SIP allows researchers to go …
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An Integrated Dataset to Understand Ecosystem Interaction
… yields a comprehensive tree and soil dataset for understanding the dynamic relationship between poplar trees and their … data set will help identify how different elements of the ecosystem interact and respond to changes in the … their genetic diversity. They hold economic value as both biofuel feedstock and their role in carbon sequestration. …
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JGI@25: A Single Cell, Myriad Microbial Discoveries
In honor of the JGI's 25th anniversary in 2022, we have revisited a number of notable achievements that showcase our collaborations and …
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New Research Sheds Light on Diversity in the Deep Sea
Microbial communities living near hydrothermal deep-sea vents hold vast potential for … get any sunlight, and yet they still support a unique array of microorganisms adapted to living in extreme conditions. … five oceanic regions. In doing so, they found a high degree of variability in the types of microorganisms that inhabit …
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Busting the Unbreakable Lignin
New evidence shows anaerobic fungi can degrade the hardiest of plant components   The Science There are three main ingredients to tackle when it comes to breaking down a … called lignocellulose, are the primary components of hardy cell walls. It’s the cellulose and hemicellulose …
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Supercharging SIP in the Fungal Hyphosphere
High-throughput stable isotope probing helps ID novel interactions between bacteria and fungi … The Science Researchers have successfully automated aspects of a commonly-used process for studying microbial … with results published in the  November 2022 issue of Microbiome . High-throughput stable isotope probing (SIP) …
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JGI Accepts 17 Proposals for Community Science Program
Monitoring Inter-Organism Interactions Within Ecosystems Latest portfolio of approved proposals highlight biodiversity and how ecosystems are adapting to stresses. A total of 17 proposals have been approved through the annual …
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When “The Blob” Made It Hotter Under the Water
Data from marine heatwave event may foreshadow climate change impact on marine microbial communities. The Science A team of researchers tracked the impact of a large-scale heatwave event in the ocean known as “The …
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