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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… applying a method that allows researchers to quickly modify diverse bacterial genomes. In 2019, the JGI’s Yasuo … domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, “Once … Pseudomonas  and Ralstonia with enzymes that make phosphate bioavailable for plants. When these engineered …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… scientists are now getting more complete genomic results from single cells. The Science Studying the  genomes of … for understanding the microbiome of the earth, but most species do not grow easily in the lab. To access the genomes … Amplification to generate enough DNA to put on a sequencer. As previously mentioned, it’s a method that’s …
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Engagement Webinar: How to Apply for Resources at Multiple DOE User Facilities
… On February 17, 2021, JGI and the Environmental Molecular … a JGI Engagement: Accessing Resources at Multiple DOE User Facilities with a Single Proposal webinar, inviting … capabilities in just one research proposal. Guest speaker Michelle O’Malley of UC Santa Barbara talked about …
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Methane Makers in Yosemite’s Lakes
… Meet researchers who have hiked, rafted and met local wildlife (a marmot!) as they’ve sampled the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains. These lakes are isolated, but …
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Inspiring STEM Careers Through a Hands-on Everglades Microbiome Study
… Journal publication caps JGI pilot project with fledgling Florida high school scientists. The Florida Everglades … Program . “After discussing the project with him, I was inspired by his enthusiasm and drive to give his students this … The U.S. Department of Energy Joint Genome Institute, a DOE Office of Science …
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Cataloging the Microbes that Manage the Health of River Systems
… Database provides details on river microbes in 90% of US continental watersheds. The Science In a recent Nature … genes to the community level. The Impact Rivers are transport systems, and their health is heavily reliant on the … program at Pacific Northwest National Laboratory. The JGI sequenced over 1,000 metagenomes and established …
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Game On: High School Students Drive Everglades Metagenomics Study
… In late June, Kathleen Lail, a member of JGI’s Sample Management Group, attended a conference in … a defined set of objectives, and started with an image of a spoonful of dirt with the question: Do you want to find out … samples to the JGI for sequencing.  While JGI processed, sequenced, and analyzed the soil samples, the JGI offered 7 …
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Researching and Solving Real-World Problems with the 2023 JGI-UC Merced Interns
… For the first time in several years, the DOE Joint Genome … (below) to hear more about the summer from their own perspectives. The interns from this cohort and their JGI … mentor Bryce Foster, Benchmarking Bioinformatics Tools for Sequence Similarity Searching Morgan Lavenstein Bendall and …
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The JGI Data Portal: Improving User Experience
… agile collaboration between the JGI and the information technology company Truss has resulted in the JGI Data Portal , a pilot download experience accessible from JGI’s plant portal Phytozome . Although the Data Portal … team’s ultimate goal is to allow scientists to search for specific types of data across organisms, enabling them to …
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New Research Sheds Light on Diversity in the Deep Sea
… living near hydrothermal deep-sea vents hold vast potential for bioenergy.   The Science Hydrothermal vents are ocean-floor openings in the Earth’s crust that emit hot, … found at each site and the role they fulfilled suggest that specific environmental conditions — like temperature, pH …
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Tracking the Origins of Methane-Producing Microbes
… Mini-Metagenomics Approach Helps Identify Novel Archaeal Methane Metabolism. Methane-producing … report the discovery of a novel archaeum from a hot spring that has the genetic machinery to perform … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 …
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Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
… A special issue of Synthetic Biology celebrates research enabled by the JGI DNA Synthesis Science Program. Fashioning DNA outside a cell is key to interrogating what … take on ambitious synthetic biology experiments. A recent special issue of the journal Synthetic Biology puts the …
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