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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
A group led by JGI scientists have made strides in improving … ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the … domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, “Once …
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Making a Lichen Together
For the first time, a team analyzes the transcriptomes of a lichen fungus and … nitrocellulose filters in Petri dishes. The scientists also sequenced the fungus and photobiont’s genomes and compared …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
With a new methodology, scientists are now getting more complete … from single cells. The Science Studying the  genomes of microorganisms is incredibly useful for understanding the … Amplification to generate enough DNA to put on a sequencer. As previously mentioned, it’s a method that’s …
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Researching and Solving Real-World Problems with the 2023 JGI-UC Merced Interns
For the first time in several years, the DOE Joint Genome Institute hosted several UC … Emerald Nash and mentor Jonelle Basso, Experimental Study of Root Colonization by Resident Phages of a Plant Growth … mentor Bryce Foster, Benchmarking Bioinformatics Tools for Sequence Similarity Searching Morgan Lavenstein Bendall and …
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Cataloging the Microbes that Manage the Health of River Systems
Database provides details on river microbes in 90% of US … program at Pacific Northwest National Laboratory. The JGI sequenced over 1,000 metagenomes and established …
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Ray Turner Completes Last Tour of Lab Duty at JGI
For the last 15 years, Ray Turner has been one constant for the Joint … as a typical monthly JGI All-Hands Meeting, after a couple of perfunctory items, the agenda went off the rails – according to plan but unbeknownst to Ray and …
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Tracking the Origins of Methane-Producing Microbes
Mini-Metagenomics Approach Helps Identify Novel Archaeal Methane … archaea are estimated to produce 500 million tons of methane a year, which is over half the total global … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 …
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Methane Makers in Yosemite’s Lakes
Meet researchers who have hiked, rafted and met local wildlife (a marmot!) as … the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains. These lakes are isolated, …
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Inspiring STEM Careers Through a Hands-on Everglades Microbiome Study
Journal publication caps JGI pilot project with fledgling Florida … school scientists. The Florida Everglades evokes images of fanboats skimming over swamps, while alligators peer through the waters and clouds of insects hover just above. Described as a “river of grass” …
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From Berkeley to Binghamton: Tracking Strawberry Evolution
In the summer of 2015, Adam Session was a postdoc working at the DOE Joint Genome … and the  oilseed crop false flax – genomes that have been sequenced by the JGI – and the common strawberry, which is …
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iPHoP: A Matchmaker for Phages and their Hosts
…   To understand virus-host dynamics, computation helps fill in what … With metagenomic sequencing, researchers have found more of these viruses than ever before, in all kinds of ecosystems. However, matching these viral genetic sequences to their hosts is crucial to understanding what …
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New Research Sheds Light on Diversity in the Deep Sea
Microbial communities living near hydrothermal deep-sea vents hold … get any sunlight, and yet they still support a unique array of microorganisms adapted to living in extreme conditions. … five oceanic regions. In doing so, they found a high degree of variability in the types of microorganisms that inhabit …
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