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When they tested the screen method on a microbial community from geothermal hot springs, they uncovered a novel … pools, followed by whole genome amplification and shotgun sequencing. The team first tested the approach on bacterial … To validate the predicted cellulases from their captured genomes, the team then used DNA synthesis and cloning, …

… single cell genomics by collecting nearly 500 single cells from a single low diversity hot spring sediment sample. … value to the other commonly used culture-independent sequencing approaches including amplicon and metagenomic … that were missed within paired metagenome assembled genomes (MAGs), and that dominant populations varied with …

And we’ve done some work with those and have a couple of publications out there on the chemistry. There’s so much … almost never Actinomycetes. And so when we look at their genomes we don’t get the same kind of information that we … really exciting work on figuring out how to get genomic sequencing data for the fungus garden because it’s more …

… calls. These documents have been shared with permission from the PIs who drafted them.  … Elucidating Sphagnum …

NOTICE Information from this server resides on a computer system funded by the U.S. Department of Energy. Anyone using this system … security personnel. … With respect to documents available from this server, neither the United States Government nor …

But SAR324 can be found throughout the water column, from the warm, well-lit surface, to the blue-lit twilight … their gene expression, the team then sequenced community genomes and expressed gene transcripts (metagenomes, … metatranscriptomes), as well as the genomes of single cells of the uncultivated SAR324. The constellation …

For accepted proposals, sequencing, synthesis, metabolomics and analysis will be paid for by the Department of Energy’s funding of the JGI. There is … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology and metabolomics capabilities …

… into how the multiple chromosomes of Miscanthus arose by ancient hybridization (polyploidy) and insights into the … genomic tools, allows Miscanthus to both inform and benefit from breeding programs of related candidate bioenergy … Understanding the evolution of polyploidy, and how genomes respond to extra chromosome and gene copies, will …

… methods for efficient QA/QC based on next-generation sequencing technologies including PacBio and Illumina.  The … on developing methods for converting digital information from sequence databases into biotechnological or … engineering pathways to function in heterologous hosts, by combining combinatorial synthesis, high-throughput …