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… “hook” of fluorescence-activated cell sorting to sort cells into several small pools, followed by whole genome … The team first tested the approach on bacterial laboratory models to set a reference for its performance, and then … cellulases from their captured genomes, the team then used DNA synthesis and cloning, followed by protein in vitro …

… An article summarizing the field of viral metagenomics, authored by JGI … Nayfach and  Nikos Kyrpides , was  recently published in the journal  Annual Review of Biomedical Data Science . … best ways to study such viruses is by sequencing the bulk DNA or RNA contained in a sample that has been collected …

… for survival, may now be easier to characterize following a proof-of-concept study in which researchers paired … to the lack of robust tools available for carrying CRISPR into microorganisms. CRAGE (Chassis-independent … employing CRAGE, researchers are no longer limited to using model host microbes; theoretically, any microbe can serve as …

… In honor of the JGI's 25th anniversary in 2022, we have … that are still vital to facilitate the assembly of small DNA fragments into larger and coherent pieces called contigs — into genes …

… A new approach for improving functional annotation in fungal genomes. The Science In … then used a series of random insertions of a foreign DNA sequence to generate a huge pool of mutants, each … mutants died and others grew well; the survivors had their DNA sequenced at the JGI as part of a 2014 Community Science …

… Igor Grigoriev from the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … multiple times in order to obtain enough zoospores. DNA and RNA samples generated from the zoospores were then … using the Illumina platform. Unfortunately, both the DNA and RNA samples turned out to be a mixture of genetic …