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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but most species … usually employ two different methods; shotgun metagenomic sequencing and single-cell sequencing. For years, extracting … stitch the pieces together and attempt to put them back into the original genomes. These metagenome-assembled …
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From Competition to Cooperation
… fungal lineages. The Science By comparing 135 fungal sequenced genomes, researchers were able to carry out a … to white rot fungi. Additionally, some of the saprotrophic genes found in the mycorrhizal species, such as nutrient transporters, were co-opted into symbiotic functions. Recently published in  Nature …
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Fishing for Novel Cellulose Degraders
… “hook” of fluorescence-activated cell sorting to sort cells into several small pools, followed by whole genome amplification and shotgun sequencing. The team first tested the approach on bacterial … The approach can be further developed to bait for specific functions other than cellulose-degradation to tease out …
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Network Modeling of Complex Data Sets
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Representing sex chromosomes in genome assemblies
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Busting the Unbreakable Lignin
… to remove lignin and transform the remaining components into  biofuels and bioproducts. If anaerobic fungi could be … JGI data portals and pipelines helped identify potential genes responsible for lignin deconstruction. (The JGI also conducted RNA sequencing and alignment to the reference fungal  genome . …
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Construction of high resolution genetic linkage maps to improve the soybean genome sequence assembly Glyma1.01
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Effects of Polar Light Cycle on Microbial Food Web
… A long-running time-series study offers a first glimpse into a full season’s cycle of this polar environment. This … more space on Earth than any other ecosystem. Therefore, understanding the microbial activity that dominates these … over a 10-year period and sent to JGI for metagenomic sequencing. A study  recently published in  Microbiome shows …
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Picking Up Threads of Cotton Genomics
… Berkeley National Laboratory (Berkeley Lab) has now sequenced and assembled the genomes of these five cotton … was to try to bring in molecular methods of breeding into cotton,” said Schmutz, who heads JGI’s Plant Program. … in this  Nature Genetics publication will facilitate deeper understanding of cotton biology and lead to higher yield and …
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Association mapping by aerial drone reveals 213 genetic associations for Sorghum bicolor biomass traits under drought
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RNA-Seq-based high-resolution linkage map reveals the genetic architecture of fruiting body development in shiitake mushroom, Lentinula edodes
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Metagenomic Sequencing of Two Salton Sea Microbiomes
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