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Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective. To … approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, … , the scientists thus set out to identify which sgRNA sequences were reliable. In an elegant, quantitative screen, …
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… and eukaryotic microbes and viruses in environmental sequence data. Dr. Schulz employs multi-omics (metagenomics, …
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