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The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal tar‐contaminated sediment, reveals physiological and metabolic versatility and evolution through extensive horizontal gene transfer
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Duplications and losses of genes encoding known elements of the stress defence system of the Aspergilli contribute to the evolution of these filamentous fungi but do not directly influence their environmental stress tolerance
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In silico analysis of the expression profile of AA9 Lytic Polysaccharide Monooxygenases (LPMOs) and the CDH Cellobiose Dehydrogenase enzyme in wood-degrader Agaricomycetes. The Pleurotus ostreatus case
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How to Target a Microbial Needle within a Community Haystack
A workflow for capturing the genetic make-up of specific uncultivated microbes … (Berkeley Lab) to develop a process for more easily identifying cells of interest in a complex environmental …
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Bacteria and Fungi Divvy Up the Work in Forest Floor
… second fiddle to those from fungi when it comes to breaking down tough plant fibers. The Science Walk in a temperate … teems beneath your feet. Both bacteria and fungi have genes that can break down plant fibers. But are they both … (transcriptomes) from their soil samples. This gene expression dataset was particularly important for …
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Uncovering Novel Genomes from Earth’s Microbiomes
… analysis leads to identification of biosynthetic gene clusters for novel secondary metabolites for multiple applications.       Despite advances in sequencing technologies and computational methods in the … genomes can’t be sequenced using traditional approaches. Identifying and characterizing the planet’s microbial …
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Whole metagenome sequencing and 16S rRNA gene amplicon analyses reveal the complex microbiome responsible for the success of enhanced in-situ reductive dechlorination (ERD) of a tetrachloroethene-contaminated Superfund site
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JGI@25: Expanding Metagenomics to Capture Viral Diversity
In honor of the JGI's 25th anniversary in 2022, we have … and a specific microbial host to replicate. Sequencing genetic material directly from samples sidesteps these … JGI researcher Antonio Camargo developed a new pipeline for identifying viruses across many thousands of genomes and …
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Extracting the Secrets of Secondary Metabolites
CRAGE-CRISPR combo helps further demystify biosynthetic gene clusters.  The Science Microbial secondary metabolites, … for survival, may now be easier to characterize following a proof-of-concept study in which researchers paired … is a technique researchers can use to integrate large genetic payloads directly into diverse microbes. Combining …
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2021 JGI Proposal Call Brings New Investigators into Community Science Program
Accepted proposals emphasize JGI interests in algae and secondary metabolites. A total of 27 … and then use computational tools to analyze biosynthetic gene clusters and then develop them for a range of … of Georgia is interested in sequencing relatives of switchgrass that are apomictic. This trait would be …
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Expanding Virophage Diversity
… are small viruses with double-stranded DNA genomes that co-infect eukaryotic cells along with giant viruses. Virophages … viruses. Almost all known virophage genomes share only four genes in common: major and minor capsid proteins (MCP and … includes  IMG/VR (for Virus)—for the virophage marker gene MCP, they were able to identify 44,221 total virophage …
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Nigel Mouncey
Dr. Mouncey joined the DOE Joint Genome Institute in 2017 as the fourth … Dr. Mouncey has a long-standing interest in microbial genetics that started with his education and carried forward … effects on dimethyl sulfoxide reductase (dor) gene expression. J Bacteriol. 180:5612-8. … AAAS Fellow (2024) …
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