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How to Submit Sequencing Samples
… Below is a brief description of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes instructions on … 1) Review JGI sample QC requirements and protocols Obtaining DNA and RNA of suitable quantity and quality has been …
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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… A group led by JGI scientists have made strides in improving and applying a method that allows researchers … the genome. “CRAGE is central to our ability to offer host-engineering for our users,” says JGI Director Nigel Mouncey. … engineered root bacteria  Pseudomonas  and Ralstonia with enzymes that make phosphate bioavailable for plants. When …
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Genome Sequence of Thermofilum pendens Reveals an Exceptional Loss of Biosynthetic Pathways without Genome Reduction▿ †
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Plant Microbial Interactions Group
… Plants are complex organisms that harbor a fantastic diversity of cell types and associated organisms, including fungal and bacterial symbionts, detrimental … approach, we employ various omics technologies, strain engineering and experimental characterization methods, in …
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Metabolomics Program
… The JGI’s Metabolomics Program works to uncover the roles of small molecules in biological systems, linking them with genomic data to … The Metabolomics Program is focused on understanding the critical roles of metabolites in living systems, particularly as they …
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JGI@25: Tracking & Subduing the Plague of California’s Oak Woodlands
… In honor of the JGI's 25th anniversary in 2022, we have … was released to the public in a highly-cited 2006 publication in the journal Science . This provided a … enables them to fend off the effects of infections. As SOD testing may be prohibitively expensive and too complex to …
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User Support
… Effective July 27, 2026, the JGI will move to a new login service, requiring the use of an ORCID account with two-factor authentication enabled … access your data, share with collaborators, and coordinate publication with JGI scientists. Synthetic DNA constructs …
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Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
… enabled by the JGI DNA Synthesis Science Program. Fashioning DNA outside a cell is key to interrogating what genes … called terpenes. Terpenes are made via a tangled network of enzymes in plants. To better pinpoint the biosynthetic … more than 10 papers, including two Nature Plants publications, in five years, have come out of the …
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Reductive amination cascades in cell‐free and resting whole cell formats for valorization of lignin deconstruction products
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Reductive Enzyme Cascades for Valorization of Polyethylene Terephthalate Deconstruction Products
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Yasuo Yoshikuni
… Dr. Yasuo Yoshikuni has been head of the DNA Synthesis Science Program since 2015. The mission of this program is to harness the power of DNA synthesis, strain engineering, and biosystems design for DOE-mission-relevant … to learn more about the program's areas of focus, major initiatives and more.  …
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Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound
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