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Institutional User Agreements
… Every approved proposal starts with a User Agreement — before any work can begin, including the submission of samples for sequencing, or the submission of sequencing data for synthesis. This agreement, required by the DOE for all … of the JGI User Agreement , JGI User Agreement for Federal Agencies , DNA Synthesis Appendix  and DNA …
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Natural Prodcast Episode 9: Roger Linington
… DAN: Welcome back for episode 9 of Natural Prodcast. This week, Alison and I … we recorded in my hotel room at SIMB, or the Society for Industrial Microbiology conference that took place in … support from the ICBG training program, which stands for International Cooperative Biodiversity Groups, and it’s …
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Resequencing and annotation of the Nostoc punctiforme ATTC 29133 genome: facilitating biofuel and high-value chemical production
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Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
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Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC
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Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping
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Boosting Small Molecule Production in Super “Soup”
… Do metabolic pathways from engineered yeasts still work in cell-free … environments? (Spoiler: Yes.) Yeast strains engineered for the biochemical conversion of glucose to value-added … alternative approaches for manufacturing bioproducts and  biofuels . This first report of their work, supported …
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Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
… PI on this project. “I view the program as an excellent platform to build collaborative research initiatives.” Yasuo … Genomics, the system allows scientists to test enzymatic pathways, which make valuable products, more quickly. In the … chemicals, such as ethanol and acetone . To help reduce production of byproducts, the JGI DNA Synthesis Science …
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Gene Expression: From On-Off Switch to Dial
… library approach to tuning gene expression in metabolic pathways. Compared to the traditional way, which leverages … approach to expression, scientists can now opt for various shades of grey. The ability to fine-tune the … pathways for making high-value bioproducts including biofuels. In addition, the team says that this experiment …
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Functional Diversity of Diterpene Synthases in the Biofuel Crop Switchgrass
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How to Submit Sequencing Samples
… of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … quantity and quality has been the rate-limiting step for many projects at the JGI. The quality of the starting … sequencing workflows and provide guidance as to how to perform sample QC prior to shipment: Importance of Sample QC …
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Characterization of four endophytic fungi as potential consolidated bioprocessing hosts for conversion of lignocellulose into advanced biofuels
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