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… and environmental challenges. In March 2008, the genome sequence of the forest fungus Laccaria bicolor was published … a JGI Flagship Genome and the first tree to have its genome sequenced. Having this first genome sequence of a mycorrhizal fungus shifted the focus from …

Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective. To … approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, … , the scientists thus set out to identify which sgRNA sequences were reliable. In an elegant, quantitative screen, …

… manipulation in the laboratory. Both germplasm (sequenced mutants and natural accessions) and protocols …