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CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria

Phylogeographic reconstruction of a bacterial species with high levels of lateral gene transfer

Divergence among Genes Encoding the Elongation Factor Tu of Yersinia Species▿

Denoising inferred functional association networks obtained by gene fusion analysis

The phenylalanine ammonia lyase (PAL) gene family shows a gymnosperm-specific lineage

Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium

Patterns of pan‐genome occupancy and gene coexpression under water‐deficit in Brachypodium distachyon

Metagenomics Reveal Dynamic Coastal Ocean Reservoir of Antibiotic Resistance Genes

Evolutionary and methodological considerations when interpreting gene presence–absence variation in pangenomes

JGI@25: From Life at the Extremes to Editing Genomes

… In honor of the JGI's 25th anniversary in 2022, we have revisited a … technology described by the Nobel Foundation as having “a revolutionary impact on the life sciences.” Building upon … nature was using it to do something important.” Related Publications: A scoutRNA Is Required for Some Type V …

An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria

… ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the … can deliver multiple types of payloads, such as individual genes, whole pathways, genome-editing tools, and reporters.” … genome editing. This means that scientists can modify gene expression, or delete genes, all with the rapid engineering …

Lawrence Berkeley National Laboratory is a U.S. DOE Office of Science
national laboratory managed by the University of California.