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Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective. To … approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, … To do this, they tested each guide in a  Y. lipolytica strain lacking a necessary enzyme for DNA repair. Whereas an …

… access to cutting-edge cell sorting, DNA amplification, and sequence analysis to recover the genomes of uncultivated … now uses laser microdissection to selectively isolate and sequence microbial aggregates after first imaging with …