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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… have made strides in improving and applying a method that allows researchers to quickly modify diverse bacterial … CRAGE, JGI has established a portfolio of microbial hosts that users can select to be engineered. “Piloting access to … demonstrated CRAGE as a versatile engineering system that allows scientists to conduct genome-wide screens and …
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An Earth Month Look at Sustainability Efforts at the JGI
… in half, relative to its 2015 baseline emissions.  Reaching that goal will mean slashing direct emissions from energy, … as well as embodied emissions. “So these are emissions that were made in creating goods and performing services,” … disposal.  To minimize emissions from the disposal end of that lifecycle, there’s a specific push going on at the JGI …
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Dealing with Drought: Uncovering Sorghum’s Secrets
… products.  Sorghum bicolor (L.) Moench is an African grass that adroitly handles droughts, floods and poor soils. This is the first paper that describes sorghum’s response to drought, from a … marginal lands not usable for conventional food crops. For that reason, its genome was sequenced by the JGI in 2009 and …
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Inspiring STEM Careers Through a Hands-on Everglades Microbiome Study
… insects hover just above. Described as a “river of grass” that stretches some 580,000 square miles across southern … in Palm Beach County, and particularly about the microbes that play roles in the methane cycle. Their data report, … they are also equipping the next generation of scientists that will help shape all of our futures.” Proposing a …
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Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
… communities of algae with warm-adapted ones, a trend that threatens to destabilize the delicate marine food web … not capable of producing all the juicy proteins and lipids that eukaryotes are.” But according to a new study published … of eukaryotes much more easily than previously suspected. “That would cause significant consequences on the entire food …
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Fungus Fuels Tree Growth
… sequenced and annotated for this study. The team found that, unlike pathogenic or mycorrhizal fungi (mutualist symbionts that induce structural changes in plant roots),  M. elongata  doesn’t have as many gene products that directly influence plant phenotype, such as secreted …
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The JGI Data Portal: Improving User Experience
… team. Based on Google Analytics data, the team noticed that a large percentage of Phytozome users who were seeking … thoughts — they’ll help us build a solid knowledge base that may inform future updates to one (or more) of the JGI … high-throughput sequencing and computational analysis that enable systems-based scientific approaches to these …
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Calculating the Costs of Multiple Switchgrass Gene Copies
… Scientists have discovered that the candidate bioenergy feedstock switchgrass has … a DOE Office of Science User Facility, has discovered that the candidate bioenergy feedstock switchgrass has … the questions they asked – and answered – in the study that recently appeared in the Proceedings of the National …
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Toward More Reproducible, Reusable Stable Isotope Probing Experiments
… Carbon-13, researchers can then safely follow the path of that modified chemical in any environment. Organisms who … and organic matter.  However, to get all of the information that SIP offers, researchers must take on a fairly … with a member of his lab, Abigayle Simpson. They found that although 20 years of SIP experiments have generated …
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SPRUCE-ing Up Science
… published their research related to how bacteria that colonize the roots of sphagnum work to transfer their … abilities. This capacity to survive elevated temperatures that normally would kill the moss may hold clues to how … expand into the world of evolutionary biology. And around that time, a collaborative internship program between the …
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Celebrating a Decade of Science through the JGI-UC Merced Genomics Internship Program
… has brought invaluable insights, thoughts, and explorations that has led to groundbreaking science and opportunities to … one. It’s proven to be a really good model for us that we try and continually replicate.”  On the other side, … “life changing” for the interns and loved the opportunities that the program provides. He mentioned working with …
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Better Genome Editing for Bioenergy
… is a powerful, high-throughput gene-editing tool that can help scientists engineer organisms for bioenergy … make the right cut, researchers created a set of guide RNAs that were effective against 94 percent of the genes in a … Yarrowia lipolytica to identify essential genes and genes that lead to increased lipid production. These insights …
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