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… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … It almost killed me when I was three. It wasn’t an MRSA strain at the time because that was in the 1980s. I’m …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … pairs. It was not linear, like Streptomyces , but circular. 10% of the genome encoded natural product pathways. Dan: … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… We focus our efforts on integrating database mining and sequence design with scaling the production of large, … developing methods for converting digital information from sequence databases into biotechnological or environmental …

… project by watching Amanda Hurley’s presentation from the 2019 JGI Genomics of Energy & Environment Meeting.] He’s also … at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

… following sites: Microbes: IMG (citation: https://doi.org/10.1093/nar/gkaa939 ) Metagenomes and Single Cells: IMG/M   (citation: https://doi.org/10.1093/nar/gkaa939 Fungi: Mycocosm (citation: … I request a culture/slant/DNA of an organism that has been sequenced at the JGI? …

… Myco-Ed has:  ~50 lineages selected for sequencing 16 genomes released 20 members from 15 institutions 300+ students 800+ iNaturalist observations … and distribution of fungal genome datasets. It includes JGI-sequenced and publicly available datasets encompassing a …

… comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes …

… Wisconsin. Like we discussed in the primer podcasts ( part 1 here , part 2 here , and part 3 here ), which you should … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …