Search

… particularly sigma factors that initiate transcription in bacteria. Using this technology, which combines an in vitro … expand the known transcriptional regulatory network in bacteria, enabling the discovery of regulatory cascades as … the 5’-ends by 5’-end sequencing, from which those promoter sequences recognized by a given sigma factor are inferred. …

… and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the … genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea.  Nature Biotechnology  35(8):725-731 … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et …

… Our research focuses on the assembly of metagenomic sequence data into microbial genomes, which is of … between two genomes,  we use it to identify and classify bacteria and archaea as well as gain insights into the … Profile HMM search across all IMG metagenomes, capturing sequence diversity for proteins of interest. …

… Bacterial enzymes play second fiddle to those from fungi … So, to be able to identify proteins, the team compiled DNA sequences (metagenomes) and messenger RNA sequences (transcriptomes) from their soil samples. This …

… 11, 2022, in Cell Genomics , the team focused on Actinobacteria ( Actinomycetota ), a group of bacteria with … Group. Many of these bacterial and archaeal genome sequences have been generated using techniques that the JGI … Over the years more than 3,400 genomes have been sequenced from curated DSMZ isolates, for which quality DNA …

… For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We … An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria Check out the agenda for the JGI’s 2021 Annual …

… And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what caves …

… a method that allows researchers to quickly modify diverse bacterial genomes. In 2019, the JGI’s Yasuo Yoshikuni and … domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, “Once … example, the JGI first used CRAGE to engineer domesticated bacterial strains with novel biosynthetic gene clusters …