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Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of Tigheibacillus jepli gen. nov., sp. nov.
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Artificial Polyploidy Improves Bacterial Single Cell Genome Recovery
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Extracting the Secrets of Secondary Metabolites
… for survival, may now be easier to characterize following a proof-of-concept study in which researchers paired CRISPR and … CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is the leading tool for accurately editing genomes, … The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science …
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JGI Portals
… The JGI’s data stewardship expertise and infrastructure enable a broad community of scientists and engineers to leverage our data to develop new algorithms, models and tools in support of DOE mission priorities. Data discoverability and … for technical help, feedback or a guided walk-through of the new Data Portal.  …
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Engagement Webinar: Utilizing long-read sequencing for metagenomics and DNA modification detection
… This webinar focused on how the JGI employs single-molecule, … to aid with genome assembly and transcriptome analysis of microbial, fungal, and plant research projects.  More … “regular” PacBio, since the low-input protocol involves PCR amplification of the library. 6mA and 4mC (~50x depth), 5mC …
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Plastome evolution in annual Brachypodium species reveals widespread heteroplasmy and chloroplast capture, lineage-specific codon usage bias, and low positive selection
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Tracing the Evolution of Shiitake Mushrooms
… New genomes highlight the diversity of white rot fungi in the genus Lentinula   The Science Shiitake mushrooms get … from the Americas to trace the evolution of Lentinula species.The Lentinula genus diverged roughly 28 million … 1456588 to D.H. and DEB-1442113 to A.R., the Department of Energy award 0000238431 to D.H., the GENOBIOREF …
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Mining Metagenomes for Cas Proteins
… Cas14 proteins discovered from JGI’s IMG/M database and biochemically … Institute.  The Science Researchers report the discovery of miniature Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated proteins that can target … The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science …
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An Integrated Dataset to Understand Ecosystem Interaction
… yields a comprehensive tree and soil dataset for understanding the dynamic relationship between poplar trees and their … data set will help identify how different elements of the ecosystem interact and respond to changes in the … surroundings — illuminating the molecular mechanisms for adaptation within the trees. The data set generated is 25 …
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SPRUCE-ing Up Science
… A former JGI intern is now applying his internship lessons on … his backyard in Tennessee — the birds are chirping and a soft breeze ripples through the trees in his backyard. … fine tune his approach. “I got a lot of that from Zhong.”   Publication: Carrell AA et al. Habitat-adapted microbial …
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The Genomes of the Fungal Plant Pathogens Cladosporium fulvum and Dothistroma septosporum Reveal Adaptation to Different Hosts and Lifestyles But Also Signatures of Common Ancestry
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Genomewide annotation and comparative genomics of cytochrome P450 monooxygenases (P450s) in the polypore species Bjerkandera adusta, Ganoderma sp. and Phlebia brevispora
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