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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… results from single cells. The Science Studying the  genomes of microorganisms is incredibly useful for … most species do not grow easily in the lab. To access the genomes of uncultured  microbes , scientists usually employ … extracting single cells and then amplifying their DNA for genome sequencing was no easy task. The traditional way of …
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Toward More Reproducible, Reusable Stable Isotope Probing Experiments
… of that modified chemical in any environment. Organisms who process the modified compound will have heavier DNA or RNA, … cycle nitrogen and carbon, or how freshwater ecosystems process methane and organic matter.  However, to get all of … proposal calls to the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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JGI@25: Cow Rumen and the Early Days of Metagenomics
… from plants is the high cost and resource requirements of processing these sturdy materials, rumen microbes and their … essentially what makes them so powerful that they can process tough cell walls.  “The JGI was really leading the … coherent pieces called contigs — into genes and even into genomes — from these environmental samples,” said Matthias …
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Forming Scientific Connections and Building Community
… This year the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … Mentors: Jessen Bredeson & Albert Wu Project: “ Pangenome de Bruijn Graph Simplification ” Peter Nguyen : … of a multiplex genomic integration system for the selection of genes essential for specialized carbon …
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Mapping the Earth’s Hidden Fungal Kingdom
… Researchers with the Department of Energy (DOE) Joint Genome Institute (JGI)’s Fungal and Algal Science Program … one of the world's most comprehensive collections of fungal genomes. A sweeping analysis published in Nature Reviews Microbiology of nearly 2,000 of these genomes reveals just how much we still have to discover …
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Streamlining Regulon Identification in Bacteria
… in Streptomyces coelicolor . The Impact As an integrative genome science user facility, connecting genes to functions … is almost impossible, and the experimental process for doing so is laborious and complex. RIViT-seq technology simplifies this identification process. Multiple factors mediate transcription, including …
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Annual Genomics of Energy & Environment Meeting
… Thank you for joining us at the 2024 DOE Joint Genome Institute (JGI) Annual Genomics of Energy & …
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JGI@25: Roots of a Mutualist Relationship
… energy and environmental challenges. In March 2008, the genome sequence of the forest fungus Laccaria bicolor was … a symbiotic relationship with the poplar, a JGI Flagship Genome and the first tree to have its genome sequenced. Having this first genome sequence of a …
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JGI@25: The Human Genome Project, or the JGI’s Origin Story
… help solve energy and environmental challenges.   The Human Genome Project began in 1990 when the U.S. Department of … studying Chromosome 5, which contains about 6% of the human genome . They discovered genes linked to conditions such as … Chromosome 19, which contains about 2% of the human genome. They were interested in genes involved in repair of …
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JGI@25: Using Team Science to Build Communities Around Data
… (Graphic: Neil Byers) The JGI launched the 1000 Fungal Genomes (1KFG) Project through a 2012 Community Science … State University led the effort to sequence 1000 fungal genomes to create two reference genomes for all fungi families — essentially, the foundation …
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Toward More Efficient Artificial Photosynthesis
… harnesses carbon captured from the atmosphere in a process that generates energy. The MPI team ultimately aims … remove carbon from the atmosphere than current natural processes and use it as a sustainable resource for making … Program (CSP) of the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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Understanding Wildfire Recovery, Starting in Soil
… the soil, a microbiome of bacteria, viruses and fungi process carbon and nitrogen, paving the way for future … to health, researchers have taken a look at microbial genomes across a spectrum of burned soils. By connecting … a window into how to model and track the forest recovery process.  Summary Most studies on post-wildfire soil …
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