Search

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … genes that are signatures for the presence of a specific type of biosynthetic gene cluster. And antiSMASH has a …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … MARC: This isn’t true of all of them, but for certain types, they’re extremely repetitive. And what that means …

… in some of the gamma-butyrolactone (GBL) and butenolide type hormones. And so, these hormones actually oftentimes … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… bacteria that work against protistsproduce, algae and other types of settlers.  DAN: Yeah you would think. Anything that … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

Overview The JGI provides sequencing, synthesis, and targeted … capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… … DAN: You’re listening to the US Department of Energy Joint Genome … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more …

… large microbes. And they have all sorts of cool phenotypes that you can visualize by your eye. So one thing I … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

Eukaryotic projects: Kerrie Barry or Vivian Ng Prokaryotic projects: … pages for details.  … Who is my project manager? … What types of products does the JGI offer? … How do I submit a … I request a culture/slant/DNA of an organism that has been sequenced at the JGI? … How do I check the current status of …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … And we know what’s in that. And you have these different types of particulate like 2.5, 10 PPMs and just the size of … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

Apple Podcasts or wherever you’re getting this and tell me why you’re listening. DAN: But, now, here’s Natural Prodcast … doing natural products isolation/structure elucidation type work. DAN: Yeah, that’s right. I want to talk about … And that’s been followed by developments in pulse sequences and improvements in the ways in which you acquire …

The Synthetic Biology lab is divided into a DNA synthesis production … We focus our efforts on integrating database mining and sequence design with scaling the production of large, … methodologies, and statistical frameworks for inferring genotype to phenotype associations. Research Team    … For …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … very expensive and very difficult. And it turns out some types of natural product pathways are really difficult to …