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… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … on synthetic biology of engineering gene expression in bacteria – however, the professor was actually quite … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… years, has been to bring generalizable technologies to bacteria, yeasts and algae previously with little to no …

… delicately above the leaf debris are revealed to be single bacterial cells, visible to the naked eye. The unusual size … focuses on marine mangrove systems, and he was looking for sulfur-oxidizing symbionts in sulfur-rich mangrove sediments … the bacterial cells on the molecular level. They amplified, sequenced and assembled the genomes. In parallel, Gros’ lab …

… in Nature and Medicine.” It’s obviously focused more on the bacterial side of the field, and it’s a little hard to find … able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … So, one of those is Acinetibacter . This is a genus of bacteria that’s really popped up at funding agencies and the …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. Not a …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … a check-up on me. And I wonder how that intersects with bacterial bioprospecting that is often done, where we’re … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … on identifying and characterizing haloperoxidases from bacteria. And with Christian, I started getting a little bit … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… in doing so enabled the JGI to be the first to publish the sequence analysis of the target chromosomes 5, 16, and 19, …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …