Search

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … clone 170/NA DNA Synthesis Constructs >10kb in size Gene clusters or pathways totaling more than 10kb in length …

… least compared to bacteria maybe?   ALISON: And how you overcame them?    NANCY:  Yeah, okay. Of course. Fungi have a … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … is that it was found that most biosynthetic genes are found clustered in the DNA. And so if you can identify a gene …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … try to find and identify natural product biosynthetic gene clusters and to do research to get a better understanding of … ALISON: Hmm. It kind of – it’s kind of like I’m smelling bacterial perfume. I mean – but it’s purposeful so it’s some …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … areas that are less of a focus, I would say. For example, marine research is not so much of a focus within BER … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … on plant natural products. Rich, rich in bacterial and marine stuff. So we need more people annotating. It will …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … papers [on] recently – on evolution of biosynthetic gene clusters. And you and I have had some arguments over beers …

… in doing so enabled the JGI to be the first to publish the sequence analysis of the target chromosomes 5, 16, and 19, …

… already had a fermentation process with already engineered strains, but this was to further build on those to try and … little bit of looking at spinosyns trying to find related clusters at Warp Drive, which I can say we didn’t. So–  … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the delineation of host-virus … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et …

… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Joint Genome … builds upon thousands of microbial genomes that have been sequenced by the JGI though not formally analyzed or … necessary to extract meaningful biological insights from sequence data, employing IMG data and tools for comparative …

… our User Program’s Community Science Program, the JGI sequenced and characterized 270 genomes of the Clostridium … could use to develop new products with their production strains. To improve the efficiency of strain engineering, LanzaTech then leveraged JGI CRAGE …