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… NIGEL MOUNCEY: And so biology and microbiology just followed me all the way through my high school days in … we did. Unfortunately, it’s not really going to see the light of day.  DAN: That’s a lot of natural products work, … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … those two years? So that’s the new model that we’re now following. And I think proposed it at a moment, and I think …

… starting to decline around the 70s. It’s really very, very low these days. So maybe one or two or three natural product … able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …

… pathway engineering Mass spectrometry based metabolomics Below is a table of standard product offerings, including a description of … sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… research is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … to identify new divergent lineages and expand the Tree of Life. We then investigate the coding potential to find …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … moving in that way similarly, but it’s moving a little bit slower. ALISON: Okay. Okay. Thank you for that insight. DAN: …

… pressed for time on this interview, so I’m hoping we can follow up with him again as the project progresses! If you’re … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … are we waking them up? Are they metabolically active and slowly ticking away at these extreme environments? I think … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… I mean, what made you want to work in natural products following him? I know a lot of people sort of veer off and go … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … bound inside the cells to tubulin, these proteins that allow the cells to divide. We found all sorts of molecules … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… joined the JGI in 2017, he brought with him 20 years of industry experience — and an opportunity to expand the JGI’s portfolio of user companies. Now the Department of Energy is uniting … high-value partnerships among members that catalyze data flows and promote novel data applications. As a large-scale …

… The Fungal & Algal Program explores the vast diversity of fungi and algae to unlock their potential for bioenergy, … and bioproducts. By sequencing and analyzing the genomes of these organisms, the program investigates the biological … into practical applications, including sustainable biofuels, biomaterials, natural products, and environmental …