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… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … a check-up on me. And I wonder how that intersects with bacterial bioprospecting that is often done, where we’re … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … of the opinion that on the geological timescale, that most bacterial natural product systems evolved, you know, a …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … I’ve seen you give a few talks on that. Can you tell us why cone snails are so cool? ERIC:  So cone snails are … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … in a microalgae. There were four genes there. Not like a bacterial gene cluster, but they were still clustered in the …

… in Nature and Medicine.” It’s obviously focused more on the bacterial side of the field, and it’s a little hard to find … able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …

… comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the … (2017) 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life.  Nature … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … And that’s what I really wanted to do. And that’s why I came back to academia. And I set that up. And we …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… computational analyses of BGC size and complexity, BGC sequence composition, metabolic similarity & compatibility, … of horizontal gene transfer. … The SMC provides complete sequence and annotation data for secondary metabolite … and the public, and provide programmatic access to all sequence and annotation data and their comments. … JGI …