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… developed at Berkeley Lab. Over the next decade, the JGI’s single cell genomics and metagenomics capabilities were harnessed in …

… genome with CRISPR-Cas9 in order to interrogate each one’s function. However, the team first needed to fix a defect in … bp) that directs the endonuclease where to cut, but these “single guide” RNAs (sgRNAs) can be faulty. Without a good … lead Cas9 to make a lethal double-strand DNA break, a dysfunctional sgRNA would be harmless. The team was thus able …