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In silico analysis of the expression profile of AA9 Lytic Polysaccharide Monooxygenases (LPMOs) and the CDH Cellobiose Dehydrogenase enzyme in wood-degrader Agaricomycetes. The Pleurotus ostreatus case
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Doubling Down on Known Protein Families
Using a novel computational approach, researchers confirm microbial diversity is wilder than ever. Imagine researchers exploring a dark room with a flashlight, … pivotal applications in biotechnology, such as DNA editing enzymes.” Leveraging Protein Families in a New Way The …
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Fishing for Novel Cellulose Degraders
A “bait and hook” single-cell genomic approach to bioprospecting. The Science One of the most vital pieces of equipment … ecologists to learn more about the diversity of microbial enzymes, which could have a plethora of potential …
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CO2 response screen in grass Brachypodium reveals the key role of a MAP kinase in CO2-triggered stomatal closure
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Plant Program
… collaborative user projects, the JGI’s Plant Program delves into the natural world’s potential to address global challenges by exploring plants’ role in capturing carbon, detoxifying soils, interacting with …
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Functional plasticity of HCO3 – uptake and CO2 fixation in Cupriavidus necator H16
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High Throughput Phosphoglycolate Phosphatase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader
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Sequencing Submission Requirements for DNA Synthesis
When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be …
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Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
… warm-adapted microbes edge polewards, they’d oust resident tiny algae. The cold polar oceans give rise to some of the … properties,” said study coauthor Igor Grigoriev , JGI Fungal & Algal Program Head. “But still, there are no … findings, like antibiotics, pharmaceuticals, and novel enzymes that function at low temperature.” said Katrin …
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MycoCosm Tutorial
Learn more about MycoCosm, a data portal with over 2,000 fungal genomes and multi-omics datasets, equipped with interactive web-based tools for comparative genomics and …
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JGI@25: From Life at the Extremes to Editing Genomes
In honor of the JGI's 25th anniversary in 2022, we have … CRISPR-Cas9 work, the Doudna lab at UC Berkeley seeks other enzymes in the CRISPR-Cas system. For the past few years … et al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science. 2018 Nov 16;362(6416):839-842. doi: …
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Gut Fungi: Unexpected Source of Novel Chemicals
Fungi from herbivore poop make surprisingly complex compounds — an energetically expensive feat … . Yet, combing through the genomes of four anaerobic fungal species has revealed, for the first time, that this … , and Piromyces finnis . In work reported in 2017, the fungal genomes were sequenced by the U.S. Department of …
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