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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… scientists usually employ two different methods; shotgun metagenomic sequencing and single-cell sequencing. For years, … that were acquired through it were usually only around 30% complete.  Described in  ISME Communications , a method … genomes from microorganisms are shotgun (group) metagenomic sequencing and single-cell (individual) …
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Special Initiatives & Programs
… support through the JGI (with the exception of DE-FOA-0003453, which is eligible for BERSS). Available Resources All JGI … refining of new technology (e.g., single-cell genomics; metagenomics, etc.).   The faculty-level scientist must be …
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Extensive Reorganization of the Plastid Genome of Trifolium subterraneum (Fabaceae) Is Associated with Numerous Repeated Sequences and Novel DNA Insertions
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Microbial Metagenomes Across a Complete Phytoplankton Bloom Cycle: High-Resolution Sampling Every 4 Hours Over 22 Days
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The GC-Rich Mitochondrial and Plastid Genomes of the Green Alga Coccomyxa Give Insight into the Evolution of Organelle DNA Nucleotide Landscape
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Decomposing a San Francisco estuary microbiome using long-read metagenomics reveals species- and strain-level dominance from picoeukaryotes to viruses
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Homoeolog expression divergence contributes to time of day changes in transcriptomic and glucosinolate responses to prolonged water limitation in Brassica napus
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Learning to Look
… use to conduct a similar type of search in microbial and metagenomic databases. In this case, the machine “learned” to … search tool combed through more than 70,000 microbial and metagenome datasets, ultimately identifying more than 10,000 … By the numbers, the new approach detected inoviruses in 3,609 (6 percent) of the 56,868 microbial genomes and 2,249 …
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How to Target a Microbial Needle within a Community Haystack
… of the microbial world through bulk community sequencing (metagenomics) and single-cell genomics, the vast majority of … of HCR-FISH+FACS for selectively enriching low abundance microbial groups from complex natural communities. … Facility, is supported under Contract No. DE-AC02-05CH11231. This study was funded by the Max Planck Society. …
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Benjamin Cole
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JGI@25: Tracking & Subduing the Plague of California’s Oak Woodlands
All told, the disease has accounted for upwards of $30 billion in losses , including the death of tens of … the SODmap Mobile app enabled SOD Blitz participants to geotag specimens they collect. Later, those are annotated with … and mechanisms of pathogenesis . Science. 2006 Sep 1;313(5791):1261-6. doi: 10.1126/science.1128796 Science News …
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Yasuo Yoshikuni
… of citramalate., Metabolic Engineering Communications , 2023 Wu et al. Structural characterization of a soil viral … - a functional chitosanase., Nature Communications , 2023 Zhao and Yoshikuni, Discovering untapped biochemistry in … Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc …
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