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JGI Early Career Researchers Featured in mSystems Special Issue
… sorting (FACS) to sort cells into several small pools before sequencing to recover rare microbes. The technique was … recent Nature Communications paper, uncovering giant virus sequences in soil for the first time. A second approach … compounds to link active microbes with specific metabolic processes. A third approach steps away from …
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Boosting Small Molecule Production in Super “Soup”
… Do metabolic pathways from engineered yeasts still work in … to make products such as proteins, and the mechanisms for maintenance to keep processes running smoothly. As … be speaking on “An Integrated Cell / Cell-free Metabolic Engineering Approach for Rapid Prototyping of Genes and …
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RECAP: Keynote Speakers Talk Innovation at 2022 JGI Annual Meeting
… The JGI was honored to host some of the brightest minds in genetics as part of our  2022 … Nobel Laureate Jennifer Doudna of the University of California, Berkeley and Berkeley Lab, who discussed the … Jill Banfield approached Doudna with repeated genetic sequences (already known as CRISPR) that came up in her …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… This single environmental trigger highlights efficiency for users requiring multiple omics methods to examine … Identifying specific genes, regulatory elements and metabolic pathways that enable that shift provides engineers … The team also identified previously unknown promoter sequences within these gene clusters, offering new …
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A More Intuitive Phytozome Interface
… of 250 plant genomes and has been under active development for over 5 years, provides users with the genome browsers, … Questions about the transition from Phytozome v12 to v13. Why is it necessary to retire Phytozome v12? The codebase … preset for the gene in question, so you can download sequences, annotations, and gene structures. These genomes’ …
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The secondary metabolism collaboratory: a database and web discussion portal for secondary metabolite biosynthetic gene clusters
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Help from Citrus’ Bitter, Hardier Cousin
… The chromosome-scale genome sequence of trifoliate orange yields clues to disease-resistance that could prove fruitful for saving Citrus from greening disease. You’ve probably … address some of the most pressing challenges of our time. For more information, please visit  …
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Fungal Friends or Foes in Plant Roots
… host health. The Science Researchers compared the genomes of fungi that colonize Arabidopsis thaliana roots with … health and diversity, which can in turn boost yields for both food and for candidate bioenergy feedstock crops. … from natural Arabidopsis populations around Europe. The JGI sequenced and annotated 41 fungal isolates from the …
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Data Discovery Tool Helps Identify Microbial Roles within Metagenomes
… Integrated Microbial Genomes and Microbiomes (IMG/M) platform — has been developed to help inform scientists and … tool to correlate protein annotations with ratios of DNA sequence fragments from the ecosystems. Selecting a protein … system results in seeing their predicted functional and metabolic roles across global metagenomes (within IMG).  The …
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Soil virus offers insight into maintaining microorganisms
… to carbon cycling.  The Science Through a collaborative effort, researchers have identified a protein in soil viruses … AMGs in soil viruses thought to break down chitin, the gene sequences were cloned by the JGI.  The JGI’s DNA synthesis … and AMGs. It could spawn future research into understanding why these AMGs exist in the first place, and what function …
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Extracting the Secrets of Secondary Metabolites
… secondary metabolites, those molecules not essential for growth yet essential for survival, may now be easier to characterize following a proof-of-concept study in which researchers paired CRISPR and … CRAGE (Chassis-independent Recombinase-Assisted Genome Engineering) is a technique researchers can use to integrate …
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Phylogenetic and Functional Analysis of Metagenome Sequence from High-Temperature Archaeal Habitats Demonstrate Linkages between Metabolic Potential and Geochemistry
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