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The Surprising Structure of a Shrub Willow Sex Chromosome
… researchers need to select and breed cultivars for the next generation. Understanding the mechanisms by which they … Laboratory and the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … the shrub willows through the  Community Science Program , using long-read sequencing to assemble the repetitive areas …
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Fishing for Novel Cellulose Degraders
… by Tanja Woyke at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … at Lawrence Berkeley National Laboratory (Berkeley Lab). Using a single-cell screen, they can now identify microbes … sorting to sort cells into several small pools, followed by whole genome amplification and shotgun sequencing. The team …
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Finding the Fermenters
… or no oxygen, life had found other ways to produce energy, using a process called fermentation. To better understand … including the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … two bacteria isolated by their lab. KBase was used to rapidly assemble and annotate the taxonomy and function of …
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Massively parallel sequencing identifies the gene Megf8 with ENU-induced mutation causing heterotaxy
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DAP-seq Drives Robust Mapping of Gene Regulation
… to technical limitations and the sheer complexity of plant genomes. Now, with improvements to DNA Affinity Purification … DAP-seq method overcame some of these challenges by using  in vitro -expressed TFs and fragmented genomic DNA, … In addition: Multiplexed barcoding enables the simultaneous profiling of multiple species; stricter filtering criteria …
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Multiple Origins and Rapid Evolution of Duplicated Mitochondrial Genes in Parthenogenetic Geckos (Heteronotia binoei; Squamata, Gekkonidae)
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Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions
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The Power of One, Amplified
… value to the other commonly used culture-independent sequencing approaches including amplicon and metagenomic … mobile genetic elements that were missed within paired metagenome assembled genomes (MAGs), and that dominant … Microscale Applications group . Cells were randomly sorted, whole genome amplified, then sequenced and assembled. Robert …
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A Model System for Perennial Grasses
… Panicum hallii genomes offer insights to drought tolerance. The Science … model to study drought tolerance in perennial grasses using Panicum hallii (Hall’s panicgrass), by generating two … near-complete genomes of P. hallii var. hallii (99.2% complete) and P. hallii var. filipes (94.8% complete) and …
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Silver age of GOLD introduces new features
… The JGI’s Genomes OnLine Database celebrates 25 years as a vital … genomics analysis initiatives. GOLD not only curates sequencing projects carried out at the U.S. Department of … a broad range of activities from proposal intake to publication. Information is also obtained and cross-checked …
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First Monoploid Reference Sequence of Sugarcane
… Sorghum genome serves as a reference for the highly complex … genomic breeding in these crops. For diploid species, sequencing programs generally focus on a genotype with two … successfully assembled a first monoploid reference sequence using a targeted approach that focused on the gene rich part …
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Enlarging Windows into Understanding Gene Functions
… four letters represents an organism’s genetic code, or genome, which underlies physical features and functions. … to a genomic DNA library. “It’s an innovative way to rapidly capture the binding location for most transcription … TFs and regulatory networks are conserved across fungi.” Using multiDAP-seq, Nagy’s team is comparing five fungal …
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