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Massive lateral transfer of genes encoding plant cell wall-degrading enzymes to the mycoparasitic fungus Trichoderma from its plant-associated hosts
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A Droplet Microfluidic Platform for Automating Genetic Engineering
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A genetic toolbox for metabolic engineering of Issatchenkia orientalis
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Leo Baumgart
… Dr. Baumgart joined the DOE Joint Genome Institute in 2018, following his … the Sequencing Technology Implementation Lead, he directs a multidisciplinary team responsible for piloting and … developing single-nuclei sequencing methods to characterize gene regulation at the level of individual cells across …
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JGI@25: Studying Sorghum’s Survival Skills
… In honor of the JGI's 25th anniversary in 2022, we have … oxygen. Both sugarcane and corn share this photosynthetic pathway. However, neither crop shares sorghum’s hardiness, … each variety stores sugars and sequesters carbon. 2015: Epigenetic Control of Drought Tolerance in Sorghum (EPICON) …
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Extracting the Secrets of Secondary Metabolites
… CRAGE-CRISPR combo helps further demystify biosynthetic gene clusters.  The Science Microbial secondary metabolites, … CRAGE (Chassis-independent Recombinase-Assisted Genome Engineering) is a technique researchers can use to integrate … in bacteria, making it difficult to identify which pathway corresponds to which metabolite. Finding a vehicle …
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What Happens Underground Influences Global Nutrient Cycles
… 2020 collaborative FICUS projects. Through the Facilities Integrating Collaborations for User Science (FICUS) program, … of the JGI’s Flagship genomes. She and her colleagues have generated a high-quality, phenotyped mutant library in … interested in conducting a controlled drought and multi-isotope labeling experiment, and the data will allow …
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Contrasting Pathways for Anaerobic Methane Oxidation in Gulf of Mexico Cold Seep Sediments
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Metaproteomics method to determine carbon sources and assimilation pathways of species in microbial communities
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Horizontal transfer of a pathway for coumarate catabolism unexpectedly inhibits purine nucleotide biosynthesis
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Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping
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Expression in poplar of dehydroshikimate dehydratase induces transcriptional and metabolic changes in the phenylpropanoid pathway
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