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… activity is focused on developing and implementing new technologies and methodologies that facilitate discovery of … Sci Rep. 2022 Nov 7;12(1):18909. Otani H, Mouncey NJ. RIViT-seq enables systematic identification of regulons of …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … Bill Fenical. And we connected to JGI through the Community Sequencing Program, and they worked with us to sequence two genomes. Those were of Salinospora tropica and …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary …

… production. They are thought to use billions of tons of the carbon dioxide trapped in biomass each year to do so. As … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 … JGI on a proposal using this approach through the Emerging Technologies Opportunity Program (ETOP). Following that …

… communities could lead to the development of new biotechnologies around clean energy,  biofuels and … nitrogen metabolism, as well as those that play a role in carbon cycling. However, the high degree of variability in … Berkeley National Laboratory, contributed metagenomic sequencing to the study as well as  genome analysis, …

… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … the JGI. And so now here at the JGI, I work with all of the sequencing data that the JGI produces to try to find and … us, we want to hear from you! We have projects in genome sequencing, DNA synthesis, transcriptomics, metabolomics, …

… [project] with JGI, how many flavin monooxygenases are you sequencing, sort of along that vein, of if you have one or … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh Alison sequenced all of these, she tried this, if you have this …

… usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain sequences of DNA, they essentially make it not possible for … PARKINSON: Exactly. We want to understand, can we utilize sequences of these TetRs to identify what molecule might …

… The Impact Despite great strides in generating genome sequences for members of the microbial world through bulk community sequencing (metagenomics) and single-cell genomics, the vast … of this protocol was enabled by the JGI’s  Emerging Technologies Opportunity Program . The ETOP helps …

… I think. DAN UDWARY: Yeah, and that was a JGI Community Sequencing Project. You’re right, yeah. NADINE ZIEMERT: Yes. … UDWARY: I think that was one of the JGI’s early moves into sequencing things for the purpose of looking at the … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … looked at these compounds through classic spectroscopic technologies, NMR, mass spec, whatnot and then we’re …