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… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary …

… production. They are thought to use billions of tons of the carbon dioxide trapped in biomass each year to do so. As … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 … JGI on a proposal using this approach through the Emerging Technologies Opportunity Program (ETOP). Following that …

… communities could lead to the development of new biotechnologies around clean energy,  biofuels and … nitrogen metabolism, as well as those that play a role in carbon cycling. However, the high degree of variability in … Berkeley National Laboratory, contributed metagenomic sequencing to the study as well as  genome analysis, …

… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … the JGI. And so now here at the JGI, I work with all of the sequencing data that the JGI produces to try to find and … us, we want to hear from you! We have projects in genome sequencing, DNA synthesis, transcriptomics, metabolomics, …

… [project] with JGI, how many flavin monooxygenases are you sequencing, sort of along that vein, of if you have one or … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh Alison sequenced all of these, she tried this, if you have this …

… usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain sequences of DNA, they essentially make it not possible for … PARKINSON: Exactly. We want to understand, can we utilize sequences of these TetRs to identify what molecule might …

… I think. DAN UDWARY: Yeah, and that was a JGI Community Sequencing Project. You’re right, yeah. NADINE ZIEMERT: Yes. … UDWARY: I think that was one of the JGI’s early moves into sequencing things for the purpose of looking at the … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … looked at these compounds through classic spectroscopic technologies, NMR, mass spec, whatnot and then we’re …

… pools, followed by whole genome amplification and shotgun sequencing. The team first tested the approach on bacterial … define as “any form of functional enrichment leading to the sequencing of genomes from either individual cells or … program (LDRD) entitled ‘Tackling microbial-mediated plant carbon decomposition using “function-driven” genomics’. …

… in macroalgae and developing the first microbial platform technologies unlocking the potential of macroalgae as an …

… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … at the time, there was maybe, yeah, several thousand fully sequenced biosynthetic gene clusters from organisms that had …

… algae in a container with access to sunlight, water and carbon dioxide so they can grow, and then harvest for use as … Researchers collaborating across multiple institutions have sequenced and analyzed the genome of an alga that thrives in … expertise at the National Labs to screen, characterize, sequence and then analyze the genomes and multi-omics …