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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… that allows researchers to quickly modify diverse bacterial genomes. In 2019, the JGI’s Yasuo Yoshikuni and his team  … ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the … engineering system that allows scientists to conduct genome-wide screens and explore biosynthetic pathway. Thus far, the …
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The Big Deal About Short Plants
… Despite their diminutive stature, “short plants” such as mosses could be uniquely powerful in helping … plant genetic sequences to what they do. But sequencing the genome of one short plant — fire moss — had an unexpected …
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How Filamentous Fungi Sense Food
… . The Science A team led by researchers at the University of California, Berkeley used a multi-omics approach to … organism for filamentous fungi, all of which contain a wide array of plant cell-wall degrading enzymes (PCWDEs) … researchers at the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User …
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Partners
… with the HudsonAlpha Institute for Biotechnology for our Plant Program, and is further leveraging the services of the Arizona Genomics Institute for DNA and RNA extractions to support plant and other efforts. The JGI works closely with DOE …
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Filling in the Plant Tree of Life
… What if we understood plants and how they adapt to their ever-changing … food, medicine, and bioenergy crops. But most available genomes are from narrow swaths of the plant tree of life. One project aims to change that. …
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Help from Citrus’ Bitter, Hardier Cousin
… The chromosome-scale genome sequence of trifoliate orange yields clues to disease-resistance that … orange ripens only bitter, intensely sour fruit. Yet this plant, native to China and now disseminated around the … The U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility …
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Bacteria and Fungi Divvy Up the Work in Forest Floor
… Bacterial enzymes play second fiddle to those from fungi … analyzing the enzymes present in the complex organic matrix of the forest floor, a team of researchers have found that … to identify proteins, the team compiled DNA sequences (metagenomes) and messenger RNA sequences (transcriptomes) from …
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Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta
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Uncovering Novel Genomes from Earth’s Microbiomes
… Large-scale comparative analysis leads to identification of biosynthetic gene clusters for novel … methods in the past decade, researchers have uncovered genomes for just a small fraction of Earth’s microbial … insights into potential applications they may have in a wide range of research fields. A public repository of 52,515 …
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The Expanding Universe of Methane Metabolisms in Archaea
… Metagenome-assembled archaeal genomes provide new insights into … gas 20 times more potent than carbon dioxide. Billions of years ago, methane-producing archaea likely played a key … “Distribution of predominant high-temperature archaeal and bacterial lineages in Yellowstone National Park,” as well as …
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Special Initiatives & Programs
… ,  Science Focus Areas  (SFA) opportunities, and the  Office of Science Early Career Research Program .   If “BERSS” is … for BERSS support through the JGI (with the exceptions of DE-FOA-0003453 and DE-FOA-0003615 , which are both … performs sequencing, metabolomics and synthesis on behalf of the U.S. Department of Energy Bioenergy Research …
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Evolution of a Fungal Gene Expression Regulator
… Describing the largest analysis to date of the modified DNA base 5mC variations in fungi. The Science Changing an A, C, T or G in a genome sequence can lead to changes in protein structures … Additionally, the team found a correlation between genome-wide 5mC abundance and the abundance of specific 5mC …
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