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… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … So I would say we run enzymatic reactions anywhere from 4 degrees to room temperature, to slightly elevated. We …

… in the field, you can probably skip straight on to Episodes 4 5 and 6, for some fun interviews, unless you want to fact … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … at MIT, and I studied microbial ecology. I studied Vibrio species in the ocean, so heterotrophic bacteria. Now, we …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … one of the most common animals in the ocean. They’re really species-diverse. I lost track actually of the number. But, … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… aflatoxin being a secondary metabolite from an Aspergillus species that is pretty toxic – can cause liver cancer and … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

Apple Podcasts or wherever you’re getting this and tell me why you’re listening. DAN: But, now, here’s Natural Prodcast … many there are to find. Conservatively, we estimate perhaps 40,000. The best we can do is take sections of the … And that’s been followed by developments in pulse sequences and improvements in the ways in which you acquire …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … able to produce chemistry that deals with reactive oxygen species and so potentially could go down that route.  DAN: … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … family would be a set of gene clusters across different species, which all encode the production of either the same …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… support through the JGI (with the exception of DE-FOA-0003453, which is eligible for BERSS). Available Resources All … capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …