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… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … that is often done, where we’re really just looking at DNA sequences, and we don’t always know necessarily or pay … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … about his move to microbiology, about drug discovery and why it’s so hard to succeed, and we talk about an ongoing … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … or you know, you’ve run into challenges that demonstrate why this is so important. BEN:  Yeah. So I wouldn’t call it …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … that I never anticipated starting– that’s not why I came to the University of Utah– is looking at the … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … between chemistry and sequence to get anywhere. Like that’s why I say the more you know, the more you know because it …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … this and that. They’re doing all this 16S and I said, “Why don’t you just include the ITS too? It’s so easy to add …

… embargo starting at construct or strain delivery.  Detailed sequence information and constructs are made publicly …