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… laboratory isolation and genome sequencing of underexplored bacterial and archaeal lineages that are important for … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … of the opinion that on the geological timescale, that most bacterial natural product systems evolved, you know, a …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … the community so far. But being here and then being at the Marine Natural Products meeting in Ventura where I got to …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … BARONA-GOMEZ: Yeah, so any metabolite, produced by bacterial or fungal metabolism by microbes, will have a … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … and so we were able to actually not dive to collect the marine tunicates, which was nice because it’s also cold. And … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … this collection in total contains approximately 100,000 bacterial strains, and a hundred thousand fungal strains. …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … areas that are less of a focus, I would say. For example, marine research is not so much of a focus within BER … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …