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… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … to less than 5kb in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … environments, from deep-sea oceanic fluids to hypersaline lakes and hyperthermal springs. This research provides … tool for generating synthetic sequencing data from genomic sequences …

… as well as different types of environments (e.g. soil, lake).  A wide range of metabolites are identified using …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … not dive to collect the marine tunicates, which was nice because it’s also cold. And so we waited until this … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … MIBiG, and newer efforts, like BiG-scape and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… and they’re very beautiful. It’s something that I’d noticed as a kid just being out in the ocean, you can see these … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… ,  Science Focus Areas  (SFA) opportunities, and the  Office of Science Early Career Research Program .   If “BERSS” … capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … number and contact information for their DOE program officer.  Submitters are strongly encouraged to contact the JGI …

… NERSC ) is to accelerate scientific discovery at the DOE Office of Science through high performance computing (HPC) and … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on … project are coordinated through JGI’s Project Management Office.  At the completion of a project, the JGI makes the …

… Udwary. I – as well as my co-host, Alison Takemura, whose voice you’ll hear in a minute – we work for the US Department … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more …

… I must’ve been around about 18. And I went to Marcel’s office. And he’s incredibly enthusiastic and gave me a bunch of … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … put into antiSMASH after downloading it was isolated from Lake Washington. So it’s really interesting that it was …

… program. MENAKA WILHELM: How cool. And I think that’s a nice segue for the next thing we wanted to ask you about, … at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …