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… For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … one of the most common animals in the ocean. They’re really species-diverse. I lost track actually of the number. But, … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … tool for generating synthetic sequencing data from genomic sequences … A predictive tool for microbial symbionts in environmental sequence data, including microbial phenotype along the …

… aflatoxin being a secondary metabolite from an Aspergillus species that is pretty toxic – can cause liver cancer and … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… through isolation and genome sequencing of new fungal species.  Students collect fungi, sterilize tissue, isolate … and distribution of fungal genome datasets. It includes JGI-sequenced and publicly available datasets encompassing a … and researchers, facilitating the transition from genomic sequences to biological inference and understanding of …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … able to produce chemistry that deals with reactive oxygen species and so potentially could go down that route.  DAN: … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … family would be a set of gene clusters across different species, which all encode the production of either the same …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … us on that. You know, as an added benefit, the S. tropica species also makes an anticancer agent that today is in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … Right. Kate Duncan: And Pseudonocardia . And we’ve got new species which we’re currently in the process of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … ecologists that they needed to consider chemical impact on species interactions, communication, that kind of thing. But … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …