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… for advice!      … The deliverables can range from raw sequence data to well-annotated assembled genomes to … The USDA, NSF, and NIH all operate sequencing programs, and potential users are urged to contact these agencies for … USDA Animal and Plant Health Inspection Service permits US Fish and Wildlife permits These examples may apply to your …

… desert? Or that certainly there was marine life, there were fishes, coral, et cetera, but they just didn’t make … Institute. And that was lovely. Because they really saw the potential. We started showing them molecules that would … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … to train new algorithms, et cetera. And that’s not just selfishly. Of course, this was also very important for the … ones? And which groups or families of gene clusters are potentially novel. So it might yield novel pathways of …

… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … and expand the Tree of Life. We then investigate the coding potential to find novel functions that may impact microbiome … 339-346 Schulz et al. (2015) Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the …

… compounds and then are defended further from being eaten by fish. And so that’s one of the things that he looked at – … you can just take a pill, right? But with a small molecule potentially, you can do that. And so we were really lucky. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… bit more into fungi and learning the differences of all the genetic differences and then expanded on on that knowledge … the University of Utah– is looking at the natural product potential of microorganisms from Great Salt Lake, which is a … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … as many different ways as possible to access the chemical potential of these or any organisms. So developing good …

Sample QC Protocol JGI’s Total RNA Sample QC Protocol JGI’s Small RNA Sample QC Protocol DNA Extraction 101 : tips, … to the JGI. PI sends Project Manager (PM) a list of organism names (or for metagenomes, sample names).  Need help … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… chemistry that deals with reactive oxygen species and so potentially could go down that route.  DAN: So the sun … products is that we don’t know what all the biosynthetic gene clusters look like.  DAN: For sure.  MARCY: We have so … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … not been previously evaluated. So we started to see this potential of looking for compounds based on these gene …

… of it. So it wasn’t only chemistry or only biochemistry or genetics. It was also ecology. It was also evolution. It was … that example, was it the case where that colleague saw a potential mechanism for why there would be this … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … this really, really interesting. Is there an opportunity potentially for them to come work in the lab and actually do …