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… are the RiPPs, which stands for “Ribosomally synthesized and Post-translationally modified Peptides.” You’ll … on this: Most of the machinery in any living organism are proteins, which are made up of chains of amino acids. … marine organisms. Except during the time that we worked in fungi. But what do you see as the overall theme of your …

So we started out internally by making a list on the whiteboard of the top 50 journals that we thought were most … could then show that to collaborators and say, “This is a prototype. If you will help, we could do this in a more … metabolomics, and natural products in plants, fungi, and microorganisms. If you want to collaborate, let …

Program supports a wide range of projects, from single-gene synthesis to the synthesis of entire chromosomes, … variations. These constructs are subsequently synthesized and tested for their ability to produce desired … and analysis tools for comparative genomics of fungi. Mycocosm enables users to navigate across sequenced …

… world. We used ships. We used land-based marine labs. We wrote cooperative grants with people in other countries. And … from the ocean. We didn’t know whether to study bacteria, fungi, archaea, the whole range of microbial life. So, we … 2,000, 5,000 different cells, different colors, different sizes, different shapes, motility, all this stuff. But when …

It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … media. So you could take International Streptomyces Protocol media number 2, ISP2, and dilute it by a factor of … 20 analogs at this point. And Casey and Nikolaou synthesized the everninomicin at Scripps, I guess, 22 years ago. …

Diversity . And there’s something called the Nagoya Protocol on access and benefit sharing. And for a very long … nothing for. We’re also working on organisms, pathogenic fungi like Candida aureus in the lab and we work on … what season they should be collected, what girth or size of the branches should be, there are all these other …

Sure. Definitely. So, I got my PhD with Paul Hergenrother at the University of Illinois at Urbana-Champaign. … oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … can activate these, utilizing some of these already synthesized molecules. DAN: OK. These receptors are coming from …

So it was great to hear her passion and enthusiasm for fungi. And we talk a lot about some of the technical … After I got my undergraduate degree, which was in general biology, I joined the Peace Corps. And when I was … asexual spore, some might be in these things we called sclerotia, which are overwintering bodies. So the complexity …

… networking, because I really enjoyed the review that you wrote with Marnix on putting that stuff together. It’s been … in the terms biosynthetic gene clusters and genome size. And they’re so complex. There’s so much still to … metabolomics, and natural products in plants, fungi, and microorganisms. If you want to collaborate, let …

I think for Natural Prodcast, I think the question that generally comes up that people come to me asking like is– … tackle more functional questions. For example, we can synthesize a gene and then figure out what it might be doing … you have questions about project relevance or experimental protocols, contact Deputy for User Programs, Tanja Woyke , …

… from which potentially natural product could be biosynthesized, then you need to develop enabling technologies. ie … penicillin from the fungal species – probably told us that fungi could be very productive sources for, say, biological … national resources. So, you may know the so-called Nagoya protocol, which prevents taking bio-resources from the native …

… chemistry, and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … that people work with are actually alpha or gammaproteobacteria. They just have this very restricted niche. …